Project MIRACLE aims to develop a real-time monitoring platform of critical analytes in organ-on-chip models by integrating multi-modal sensors into a disposable add-on container based on optical fluorescence quenching and impedance spectroscopy. The proposed solution will integrate optical sensing for oxygen, glucose, and pH, as well as impedance spectroscopy for tissue integrity monitoring. Optical sensors will be embedded in a single-use container, providing an efficient and scalable platform for advanced monitoring without compromising the physiological relevance of the model. The impedance spectroscopy unit will be integrated into the manifold of Cellbox Labs instrument thus providing a comprehensive next generation organ-on-chip platform. This project will address critical gaps in current organ-on-chip technology, including the absence of real-time monitoring for vital parameters that significantly affect cellular viability and experimental outcomes. 

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