Project MIRACLE aims to develop a real-time monitoring platform of critical analytes inorgan-on-chip models by integrating multi-modal sensors into a disposableadd-on container based on optical fluorescence quenching and impedancespectroscopy. The proposed solution will integrate optical sensing for oxygen,glucose, and pH, as well as impedance spectroscopy for tissue integritymonitoring. Optical sensors will be embedded in a single-use container,providing an efficient and scalable platform for advanced monitoring withoutcompromising the physiological relevance of the model. The impedancespectroscopy unit will be integrated into the manifold of Cellbox Labsinstrument thus providing a comprehensive next generation organ-on-chipplatform. This project will address critical gaps in current organ-on-chiptechnology, including the absence of real-time monitoring for vital parametersthat significantly affect cellular viability and experimental outcomes. 

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